dna damage comet assay kit  (Beyotime)

Journal: Cell Death and Differentiation

Article Title: USP18 promotes nasopharyngeal carcinoma radioresistance via TRIM29 oligomerization and ubiquitination

doi: 10.1038/s41418-025-01615-3

Figure Lengend Snippet: A Heatmap of differentially expressed proteins ( | fold change | > 1.5 and p < 0.05) identified by proteome sequencing in five paired NPC biopsy tissues from patients with (irradiation-resistant) or without (irradiation-sensitive) relapse after radiotherapy. B Venn diagram showing common proteins between differentially expressed proteins identified by proteome sequencing and DUBs from the iUUCD database. C Normalized protein expression level of USP18 in these five paired NPC tissues. D RT-qPCR analysis of relative USP18 mRNA expression in NP69 and various NPC cell lines (SUNE1, HONE1, HK-1, S18, 5-8 F, 6-10B and CNE1). E Western blot analysis of USP18 and α-Tubulin protein expression in NP69 and NPC cell lines, along with normal (N) and NPC (T) tissues. F GSEA of the GSE102349 dataset revealed enrichment of radiation response-related and DNA damage response pathways in the USP18 high-expression group. G Clonogenic assays and survival fraction curves of USP18-KO or NC HK1 cells after exposure to varying IR doses. H Flow cytometry analysis of apoptosis rates in USP18-KO or NC HK1 cells with or without 8 Gy IR exposure for 48 h. Representative images and quantitative analysis of γH2AX foci I , comet assay and tail moments J in USP18-KO or NC HK1 cells treated with 8 Gy IR for 0 h, 0.5 h and 4 h. Scale bars, 10 μm, n = 10 I . Scale bars, 100μm, n = 20 J . Macroscopic images K , average tumor volume L , and average weight M of excised tumors in each group ( n = 6). Data are presented as mean ± SD in D, G to J, L and M ; * p < 0.05, ** p < 0.01, *** p < 0.001; p values were determined using two-way ANOVA; n = 3 independent experiments. The unprocessed images of the blots are shown in Fig. S .

Article Snippet: A Comet Assay DNA Damage Detection Kit (Keygen Biotech) was employed.

Techniques: Sequencing, Irradiation, Expressing, Quantitative RT-PCR, Western Blot, Flow Cytometry, Single Cell Gel Electrophoresis

Journal: Cell Death and Differentiation

Article Title: USP18 promotes nasopharyngeal carcinoma radioresistance via TRIM29 oligomerization and ubiquitination

doi: 10.1038/s41418-025-01615-3

Figure Lengend Snippet: A, B Western blot analysis (anti-Flag, TRIM29, and α-Tubulin) in SUNE1 and HK1 cells stably expressing vector, USP18-Flag, or USP18-Flag plus shTRIM29. C, D Representative images and quantitative analysis of γH2AX foci in SUNE1 and HK1 cells treated with 8 Gy IR for 0, 0.5, or 4 h. Scale bars: 10 μm, n = 10. E, F Representative comet images and quantitative analysis of tail moments for DNA damage in SUNE1 or HK1 cells treated with 8 Gy IR for 0, 0.5, or 4 h. Scale bars: 100 μm, n = 20. G Flow cytometry analysis of apoptosis rates in SUNE1 or HK1 cells exposed to 8 Gy IR. H Clonogenic assays of SUNE1 and HK1 cells following exposure to indicated IR doses. Macroscopic images I , average tumor volume J , and average weight K of excised tumors in each group ( n = 6). Data are presented as mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, with p values determined using two-way ANOVA; n = 3 independent experiments. The unprocessed images of the blots are shown in Fig. S .

Article Snippet: A Comet Assay DNA Damage Detection Kit (Keygen Biotech) was employed.

Techniques: Western Blot, Stable Transfection, Expressing, Plasmid Preparation, Flow Cytometry